The field of bioinformatics emerged as a tool to facilitate biological discoveries more than 10 years ago. Bioinformatics is an interdisciplinary area of the science composed of biology, mathematics and computer science. Bioinformatics is the application of information technology to manage biological data that helps in decoding plant genomes. With the development of Human Genome Project (HGP), the field of Bioinformatics expanded very rapidly since the ability to capture, manage, process, analyze and interpret data became more important than ever. Bioinformatics and computers can help scientists to solve medical problems by interpreting large quantum of data available from experiments. The need for time to complete experiments came down drastically, thus facilitating new medical breakthroughs. Application of various bioinformatics tools in biological research enables storage, retrieval, analysis, annotation and visualization of results and promotes better understanding of biological system. This has greatly assisted in human, animal and plant disease diagnosis and treatment.